-20°C
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (> 5kb). M-MLV RT is the preferred reverse transcriptase for long mRNA templates because the RNase H activity of M-MLV RT is weaker than the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Source: Recombinant expressed in E. coli
Unit Definition: One unit is defined as that amount of enzyme required to catalyze the transfer of 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3), 7mM MgCl2, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [3H] dTTP, 0.025mM oligo(dT50), 0.25mM poly(A)400 and 0.01% NP-40.
0.1 lbs
Research or further manufacturing use only, not for food or drug use.
